Under physiological condition, most protein-protein interactions are transient, and happen in a very short duration, which make them difficult to be studied. Crosslinking reagents, or crosslinkers, provide the analytical solution to capture protein-protein complexes by covalently binding them together as they interact, to freeze even transient, weak interactions for consequent isolation and characterization. protein crosslinking https://www.creative-proteomics.com/services/crosslinking-protein-interaction-analysis.htm
